Gain-of-function mutation in the KCNMB1 potassium channel subunit is associated with low prevalence of diastolic hypertension
J. Clin. Invest. José M. Fernández-Fernández, et al. 113:1032 doi:10.1172/JCI20347 [Go to this article.]

Figure 5
Effect of β_1E65K on BK channel activation and deactivation kinetics. (A) Current traces normalized to peak current were obtained with a pulse from 0 mV to +200 mV in the presence of 1.6 μM Ca²⁺. (B) Activation-time constants (at 1.6 μM Ca²⁺) were fitted with a single exponential function and plotted versus the pulse potential for α (open triangles, n = 4), α+β_1WT (open circles, n = 13), α+β_1E65K (filled circles, n = 15), and α+β_1WT+β_1E65K currents (open squares, n = 6). (C) Plot of activation-time constants versus Ca²⁺ concentrations measured with a pulse to +200 mV. (D) Families of tail currents recorded at 1.6 μM Ca²⁺ under the four conditions. (E and F) Deactivation-time constants were obtained by fitting of the tail currents with a single exponential function and plotted versus the pulse potential (E) or versus the Ca²⁺ concentration at –80 mV (F).