Altered lipid raft–associated signaling and ganglioside expression in T lymphocytes from patients with systemic lupus erythematosus
J. Clin. Invest. Elizabeth C. Jury, et al. 113:1176 doi:10.1172/JCI20345 [Go to this article.]

Figure 2
Altered association of CD45 with LR domains in T cells from patients with SLE. Purified T cells from 12 patients with SLE and 8 healthy controls were examined as described in Figure 1 for CSK, c-Cbl, and CD45. (A–C) Representative experiment showing colocalization of CSK (A), c-Cbl (B), and CD45 (C) to LR domains in SLE and normal T cells (Normal). Scale bars, 5 μm. (D) Quantitative results showing the percent of CD3⁺ cells displaying areas of CSK, c-Cbl, and CD45 colocalization with lipid rafts in patients with SLE and healthy controls (Normal). On average, 50 cells were analyzed for each individual sample. (E) Correlation between CD45 and LCK colocalization with rafts domains in individual samples; on average, 50 cells were assessed from each patient or control. T cells from normal controls (top panel), patients with SLE with an increased CD45/LR association (middle panel), and SLE patients with normal CD45/LR colocalization (bottom panel).