The IL-12Rβ2 gene functions as a tumor suppressor in human B cell malignancies
J. Clin. Invest. Irma Airoldi, et al. 113:1651 doi:10.1172/JCI20303 [
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Figure 3Methylation analysis of nepolastic B cells versus their normal counterparts. (
A) Top panel: Plot of the expected versus observed CpG dinucleotides frequency surrounding exon 1 of the
IL-12Rβ
2 gene. The CpG island and exon 1 are indicated. The broken line indicates the cut-off value of 0.60. Bottom panel: MSP analysis of cells expressing (Th1 clone, unfractionated tonsil B cells, and purified naive, GC, and memory B cells) and not expressing (RPMI 8866 LCL and Raji, BRGM, and Daudi BL cells) the
IL12Rβ
2 gene. Cells expressing the gene show only the amplification band corresponding to the unmethylated sequence (UM), whereas in nonexpressing cells, this band is present only in trace amounts, and the major product of amplification corresponds to the methylated (M) target sequence. (
B) Induction of
IL-12Rβ
2 gene expression in Raji and RPMI 8866 cell lines treated with 5-Aza-2′-deoxycytidine. One experiment representative of the five performed is shown. From left to right: molecular weight markers; B cells cultured with medium alone (medium) or in the presence of 5ΒM 5-Aza-2′-deoxycytidine for 24 to 96 hours.
