Specifically activated memory T cell subsets from cancer patients recognize and reject xenotransplanted autologous tumors
J. Clin. Invest. Philipp Beckhove, et al. 114:67 doi:10.1172/JCI20278 [Go to this article.]

Figure 3
Characterization of tumor-infiltrating cells after ADI. Breast cancer or normal skin specimens from three patients were xenotransplanted into five mice. (A) Staining with mAb against CD3 21 days after transfer of autologous memory or naive T cells into tumor-bearing mice. (B) Phenotype of tumor-infiltrating memory T cells 21 days after their transfer into tumor-bearing mice. Tumor tissue was stained with mAb’s against CD4, CD8, CD45R0, and CD45RA. Slides from A and B were counterstained with hemalaun. (C) Colocalization of transferred DCs (CD11c, dark blue; arrows) and T cells (red; arrowheads) within tumor tissue 21 days after adoptive transfer. Original magnifications, ×250 (A and B) and ×400 (C). (D) Numbers of CD3⁺ T cells and CD11c⁺ DCs in tumor or skin transplants determined by immunohistochemistry at day 9 and day 21 after ADI (black bars) and from untreated mice (white bars). Tumor and skin transplants from untreated mice (white bars) were used as a control. Means ± SD from three independent experiments (specimens from three patients transplanted into five mice) are depicted. Three sections per transplant and mouse were analyzed. *P < 0.05, significant differences compared with corresponding untreated controls.