Defects in secretion, aggregation, and thrombus formation in platelets from mice lacking Akt2
J. Clin. Invest. Donna Woulfe, et al. 113:441 doi:10.1172/JCI20267 [Go to this article.]

Figure 7
Akt phosphorylation in platelets from Gq–/– and Gi2–/– mice. Washed platelets were obtained and incubated with buffer alone, ADP (10 μM), thrombin (1 U/ml), or thrombin + LY294002 (50 μM) using the protocol described in Figure 1, then immunoblotted with an Ab specific for phosphorylated Akt serine 473. The results shown are representative of those obtained four times each.