Defects in secretion, aggregation, and thrombus formation in platelets from mice lacking Akt2
J. Clin. Invest. Donna Woulfe, et al. 113:441 doi:10.1172/JCI20267 [Go to this article.]

Figure 3
Secretion in WT versus Akt-deficient platelets. (a) Dense-granule secretion. Release of (3H)5-HT was determined after incubation with the indicated concentrations of AYPGQV. The results shown are the mean ± SEM of three experiments, *P < 0.05 (Student’s t test). (b) Platelet α-granule secretion. Washed human platelets were stimulated with buffer alone (black line), 250 μM AYPGQV (b blue line), 500 μM AYPGQV (pink line), or 1M AYPGQV (light blue line), stained with FITC-labeled anti–P selectin Ab, and analyzed by flow cytometry. Similar results were obtained in an additional two experiments. FL1-H, fluorescence intensity.