Human immunoglobulin selection associated with class switch and possible tolerogenic origins for Cδ class-switched B cells
J. Clin. Invest. Nai-Ying Zheng, et al. 113:1188 doi:10.1172/JCI20255 [Go to this article.]

Figure 3
The autoimmune-associated J_H6 gene is counterselected from IgG populations and selected into the Cδ-CS lineage. (A and B) The J_H4 gene segment is normally the dominant J_H gene used in human V_H gene rearrangements. The J_H6 gene is upregulated in receptor-edited and autoimmune B cells, linking it to autoimmune repertoires. Only usage of J_H4 versus J_H6 gene segments is shown and compared, as all other J_H gene segment use is unchanged (other J_H representations are in Table 2). J_H analysis was based on naive B cells (ten donors), IgM/D⁺ pre-GC cells (three donors), IgM⁺ GC cells (eight donors), IgM⁺ memory cells (ten donors), IgM⁺ PCs (two donors), IgG⁺ GC cells (seven donors), IgG⁺ memory cells (eight donors), IgG⁺ PCs (two donors), Cδ-CS PCs (one donor), and Cδ-CS GC cells (ten, five, and four donors for V_H4, V_H1, and V_H3 libraries, respectively). Bars indicate means of all donors and error bars, the SD. Student’s t test P values are indicated. *P < 0.0005 vs. all; **P < 0005; ^#P < 0.01; ^##P < 0.0001.