Isoform-specific regulation of mood behavior and pancreatic β cell and cardiovascular function by L-type Ca²⁺ channels
J. Clin. Invest. Martina J. Sinnegger-Brauns, et al. 113:1430 doi:10.1172/JCI20208 [Go to this article.]

Figure 2
Biophysical properties of Ca_v1.2 currents in Ca_v1.2DHP–/– cardiomyocytes. (A and B) I_Ba through LTCCs was recorded from cardiomyocytes isolated from neonatal WT or homozygous mutant mice. Currents recorded with no treatment (CO) or 3 minutes after perfusion with 1 ∝M isradipine (ISR 1) or 0.1 ∝M BayK (BayK 0.1) and 1 ∝M BayK (BayK 1). Holding potential, –80 mV; test potential, +10 mV. One of more than three experiments yielding similar results is shown. (C) Concentration-dependent isradipine inhibition. Each curve was constructed from experiments (n = 5) as described in A. (D) Time constants (τ) for current inactivation during 300-ms depolarizations from –80 mV to different test potentials were obtained by fitting the inactivation data to a monoexponential function. No statistically significant difference was found between myocytes from WT and mutant mice.