Expression of c-kit in NSPCs in vitro and in vivo. (A) Expression of c-kit mRNA in human NSPCs (hNSPC) and mouse NSPCs (mNSPC), as measured by semiquantitative RT-PCR. (B and C) Immunofluorescence analysis for c-kit and nestin in mouse (B) and human (C) NSPCs. (D and E) Expression of c-kit in NSPCs in vivo. (D) Double staining of mouse brain by c-kit and nestin revealed c-kit expression in nestin-positive cells (arrows) in the SVZ. (E) Representative orthogonal images of colocalization of c-kit and nestin in vivo. Upper, main, and left panels show views of the xz, xy, and yz planes, respectively. Lines represent coordinates in each plane. x axis, 24.4 ∝m; y axis, 24.4 ∝m; z axis, 6.7 ∝m; optical section thickness, 0.02 ∝m. Scale bar (shown in B): B_D, 20 ∝m.