Cholesterol targeting alters lipid raft composition and cell survival in prostate cancer cells and xenografts
J. Clin. Invest. Liyan Zhuang, et al. 115:959 doi:10.1172/JCI19935 [Go to this article.]

Figure 3
Cholesterol depletion inhibits Akt phosphorylation but does not induce apoptosis in PrECs. (A) Immunodetection of Akt in cell lysates following treatments. PrECs were treated with 20 ng/ml EGF, in the presence or absence of cholesterol complexes, under the following conditions: 1 hour of pretreatment with 2 μg/ml filipin (lane 2), 1 hour of pretreatment with 20 mM cyclodextrin (lane 3), 16 hours of pretreatment with 20 μM simvastatin (lane 4), 1 hour of pretreatment with vehicle (lane 5). After 1 hour of cholesterol pretreatment (lane 6), some groups were incubated with 2 μg/ml filipin (lane 7) or 20 mM cyclodextrin (lane 8). Other groups treated identically to conditions in lanes 7 and 8 were repleted (asterisk) with cholesterol for 1 hour (lanes 9 and 10). (B) PrECs were treated with varying concentrations of simvastatin or vehicle for 24 hours, and the extent of apoptosis was determined by DNA fragmentation. Values shown are means ± SD of triplicate determinations.