Targeted deletion of BMK1/ERK5 in adult mice perturbs vascular integrity and leads to endothelial failure
J. Clin. Invest. Masaaki Hayashi, et al. 113:1138 doi:10.1172/JCI19890 [Go to this article.]

Figure 5
Analysis of apoptotic changes in BMK1-CKO mice. (A–D) Ultrastructure analyses of capillaries in BMK1-CKO mice. Transmission electron microscopy was performed in the capillaries of control heart (A) and BMK1-CKO heart (B−D). (E and F) TUNEL and CD31 immunohistochemical staining of heart sections. Confocal immunofluorescent analysis of heart sections stained with CD31 (red) and TUNEL (green) from control mice (E) and BMK1-CKO mice (F). (G) Quantitative analysis of EC apoptosis. Values are mean ± SEM. Asterisk indicates electron-lucent EC. Cm, cardiomyocyte; E, erythrocyte; L, lumen; P, pericyte. Arrow indicates the fenestration of the capillary. Scale bars: A−D, 2 μm; E and F, 10 μm.