Targeted deletion of BMK1/ERK5 in adult mice perturbs vascular integrity and leads to endothelial failure
J. Clin. Invest. Masaaki Hayashi, et al. 113:1138 doi:10.1172/JCI19890 [Go to this article.]

Figure 1
Conditional gene targeting of the BMK1 gene. (A) Diagram of the WT BMK1 genomic locus (+), the targeted BMK1 allele (flp), the floxed BMK1 allele (flox), and the BMK1 null allele (–). Open and closed arrowheads indicate FRT and loxP sites, respectively. (B) Southern blot analysis of targeted ES cell clones. DNAs were digested with XhoI and hybridized with external 5′ probe or internal Neo probe. M, marker. (C) Efficiency of Mx1-Cre–mediated BMK1 deletion in various organs. Hybridization signals from Southern blot analysis were quantified, and the deletion efficiency was calculated as the percentage of signals of deleted alleles in the total signals. BM, bone marrow. (D) Immunoblot analysis of BMK1 protein in the livers from pIpC-treated BMK1^flox/flox, BMK1^flox/+, and BMK1^+/+ mice carrying or not carrying the Mx1-Cre transgene as indicated. (E) Mortality of BMK1-CKO mice after pIpC treatment: n = 12 for BMK1^flox/+ mice; n = 20 for BMK1^flox/flox mice; n = 20 for BMK1^flox/– mice.