In situ hybridization of human and mouse TNDM locus genes in pancreatic islets. Cryostat sections of pancreas from TNDM29 (A–F) and wild-type (G–J) mice of the indicated postnatal ages hybridized with antisense probes for human ZAC (A, C, and I), mouse Zac (E, G), human HYMAI (B, D, and J) and mouse Hymai (F and H). Arrows indicate pancreatic islets; arrowheads indicate exocrine parts. Note that the labeling for human ZAC and HYMAI is heavier in islets and weaker in the exocrine pancreas (A–D), whereas the opposite is true for Zac and Hymai (E–H). (I and J) Hybridization of human ZAC and HYMAI probes to nontransgenic sections as negative control. Scale bars: 70 μm (A and B); 90 μm (C–J).