CBS domains form energy-sensing modules whose binding of adenosine ligands is disrupted by disease mutations
J. Clin. Invest. John W. Scott, et al. 113:274 doi:10.1172/JCI19874 [Go to this article.]

Figure 4
(a) Activation of recombinant α1β1γ2 heterotrimers, with or without WPWS mutations, by AMP. (b) Activation of recombinant α1β1γ1, α1β1γ2, and α1β1γ3 heterotrimers by AMP. (c) Activation of α1β1γ2 heterotrimers, with or without WPWS mutations, by slow versus rapid lysis. Plasmids expressing myc-tagged α1 and β1 plus one of the subunits γ1, γ2 (with or without WPWS mutations), and γ3 were expressed in CCL13 cells, and the recombinant complexes were immunoprecipitated using anti-myc antibodies. AMPK activity was then determined at various concentrations of AMP. In a and b, the cells were harvested by slow lysis to elicit maximal phosphorylation (32); in c, the cells were harvested by rapid or slow lysis and the assays were conducted at 200 μM AMP. Results are means ± SE for duplicate immunoprecipitations.