CBS domains form energy-sensing modules whose binding of adenosine ligands is disrupted by disease mutations
J. Clin. Invest. John W. Scott, et al. 113:274 doi:10.1172/JCI19874 [Go to this article.]

Figure 1
Binding of AMP (a and c) or ATP (b and d) by the CBS1-2 construct (a and b) or the CBS3-4 construct (c and d). The fusion proteins were either the WT (open circles) or one of five point mutants (R302Q, filled circles; Lins, open squares; H383R, filled squares; T400N, open triangles; R531G, filled triangles). Data were fitted to a single-site binding model: bound = [nucleotide]/(K_d + [nucleotide]). The curves are theoretical curves obtained using the K_d values shown in Table 1.