Nkx2-5 mutation causes anatomic hypoplasia of the cardiac conduction system
J. Clin. Invest. Patrick Y. Jay, et al. 113:1130 doi:10.1172/JCI19846 [Go to this article.]

Figure 3
Hypoplasia of the peripheral conduction system in Nkx2-5^neo/+ mice revealed by minK-lacZ expression. (A) In E14.5 hearts minK-lacZ enzymatic activity is proportional to minK-lacZ (copy numbers 0, 1, or 2) and Nkx2-5 gene dosage. Whole-mount images of minK-lacZ–stained neonatal hearts contrast the dense Purkinje fiber network in WT (B and D) compared with the hypocellular system in Nkx2-5^+/neo mice (C and E). Sections show that the prominent blue X-gal stain shown in the whole mount is the peripheral conduction system at the interventricular septum (D and E). The hearts shown were from minK-lacZ homozygotes; heterozygotes yield similar results. The images shown are representative of at least three hearts. P < 0.05.