CD4⁺CD25⁺ regulatory T cells suppress allograft rejection mediated by memory CD8⁺ T cells via a CD30-dependent mechanism
J. Clin. Invest. Zhenhua Dai, et al. 113:310 doi:10.1172/JCI19727 [Go to this article.]

Figure 3
CD30 expression on Treg cells is critical for their suppression of allograft rejection mediated by memory CD8⁺ T cells. Splenectomized aly mice, transplanted 1 day earlier with either BALB/c skin (a) or heart (b) allografts, received 1 × 10⁶ WT (open squares, n = 8) or CD30^–/– (open circles, n = 6) memory CD8⁺ T cells alone, WT memory CD8⁺ T cells (M) plus WT Treg cells (filled squares, n = 8), M plus CD30^–/– Treg cells (filled circles, n = 8), M plus anti-CD30L Ab (filled triangles, n = 5), and M plus both WT Treg cells and anti-CD30L Ab (open triangles, n = 5). Suppression of rejection is diminished when Treg cells lack CD30 (filled squares vs. filled circles, P < 0.05). (c) The same mice received 1 × 10⁶ WT memory CD8⁺ T cells (M) (open squares, n = 8) alone or in combination with Treg cells generated in WT mice that also received anti-CD30L Ab (filled diamonds, n = 5) or isotype Ab (open diamonds, n = 5) at the time of DST treatment for Treg cell induction. (d) Treg cells also suppress CD8⁺ memory recall in WT hosts. WT B6 mice, transplanted 1 day earlier with BALB/c heart allografts, received WT naive (open triangles, n = 6) or WT memory (open squares, n = 6) CD8⁺ cells alone, WT memory CD8⁺ cells (M) plus Treg cells from naive (open circles, n = 5) or DST-treated (filled squares, n = 8) WT mice, and M plus DST-treated CD30^–/– Treg cells (filled circles, n = 8).