CD4⁺CD25⁺ regulatory T cells suppress allograft rejection mediated by memory CD8⁺ T cells via a CD30-dependent mechanism
J. Clin. Invest. Zhenhua Dai, et al. 113:310 doi:10.1172/JCI19727 [Go to this article.]

Figure 1
Antigen-induced Treg cells suppress allograft rejection mediated by memory CD8⁺ T cells. (a) Splenectomized aly mice (H-2^b), transplanted 1 day earlier with BALB/c skin grafts (H-2^d), received 1 × 10⁶ naive CD8⁺ T cells from WT B6 mice (H-2^b) (open triangles, n = 5) or 2C mice (open diamonds, n = 5); or 1 × 10⁶ memory CD8⁺ T cells from WT B6 mice immunized with donor-specific splenocytes (H-2^d) (squares, n = 8) or with third-party splenocytes (H-2^k) (open inverted triangles, n = 6), or from 2C mice immunized with donor-specific splenocytes (filled diamonds, n = 6). (b) The same splenectomized aly mice received 1 × 10⁶ Treg cells from naive (open circles, n = 5) or DST-treated (filled circles, n = 5) WT mice either alone or in combination with donor-specific memory CD8⁺ T cells (M + naive Treg, filled triangles, n = 6; or M + DST Treg, filled squares, n = 8). Control mice received donor-specific memory CD8⁺ T cells either alone (open squares, n = 8) or in combination with Treg cells from TPT-treated mice (M + TPT Treg, crossed circles, n = 5). DST-induced Treg cells suppressed CD8⁺ memory T cell–mediated skin allograft rejection (filled vs. open squares, P < 0.05).