Salicylate induces an antibiotic efflux pump in Burkholderia cepacia complex genomovar III (B. cenocepacia)
J. Clin. Invest. Bindu M. Nair, et al. 113:464 doi:10.1172/JCI19710 [Go to this article.]

Figure 3
Effect of growth conditions on ceoR promoter activity. Effects of the concentrations of iron (a), salicylate (b), and chloramphenicol (c) on ceoR promoter–driven expression of β-galactosidase using a ceoR-lacZ fusion, pDN19lacΩpceoR (black bars), compared with pDN19lacΩ without the ceoR promoter region (gray bars). The host strain was PC121, which secretes salicylate in response to iron starvation and demonstrates salicylate-inducible resistance to chloramphenicol, trimethoprim, and ciprofloxacin. For the iron titration, it was necessary to perform the experiment on 3 different days; thus, the data were normalized for comparison. The salicylate and chloramphenicol experiments were performed in triplicate on the same day, thus normalization was not required. Each data point shows the mean and SD.