Salicylate induces an antibiotic efflux pump in Burkholderia cepacia complex genomovar III (B. cenocepacia)
J. Clin. Invest. Bindu M. Nair, et al. 113:464 doi:10.1172/JCI19710 [
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Figure 1The
ceo operon was analyzed by RT-PCR to assess cotranscription of
llpE,
ceoA,
ceoB, and
opcM. The order of the structural genes of the
ceo operon as well as the positioning of the primers used in cDNA synthesis and in PCR amplification are shown on top. Each PCR amplification also included amplification of cDNA synthesized as a control without reverse transcriptase to ensure that all amplification was a result of cDNA and not contaminating DNA molecules; a representative result is shown in lane A. Lanes B, C, and D show amplification of
llpE,
ceoA, and
ceoB, respectively. The appearance of expected bands in the three sets of PCR reactions impies cotranscription of
llpE,
ceoA,
ceoB, and
opcM.*No reverse transcriptase; this experiment was performed for all reactions and was negative, but data are shown only for the llpE reaction.
