Lamin A/C deficiency causes defective nuclear mechanics and mechanotransduction
J. Clin. Invest. Jan Lammerding, et al. 113:370 doi:10.1172/JCI19670 [
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Figure 1Nuclear mechanics is impaired in lamin A/C–deficient cells. (
a) Nucleus of WT fibroblast before strain (red) and at 22% strain (yellow). Scale bar: 10 μm. (
b)
Lmna–/– nucleus before strain (red) and at 19% strain (yellow). Scale bar: 10 μm. (
c) Nuclear strain as a function of applied membrane strain. Dashed lines represent linear regression of the data for each cell type forced through the origin (
Lmna+/+:
y = 0.299
x,
Lmna–/–:
y = 0.626
x). (
d) Maximal normalized nuclear strain was significantly increased in
Lmna–/– fibroblasts (0.306 ± 0.029 vs. 0.626 ± 0.039;
P < 0.0001,
n = 21).
