Possible role of autoantibodies in the pathophysiology of GM2 gangliosidoses
J. Clin. Invest. Akira Yamaguchi, et al. 113:200 doi:10.1172/JCI19639 [
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Figure 4Analysis of ganglioside storage. (
a) Immunohistochemical detection of GM2 in the cerebral cortex of a 14-week-old mouse. Both
Hexb–/–FcRγ+/+and
Hexb–/–FcRγ–/– mice, but not WT (
Hexb+/+FcRγ+/+), showed GM2 storage in the cerebellum (stained green or yellow). PI was used for nuclear staining. (
b) Extraction and analysis of brain gangliosides. Gangliosides were extracted from the brains of 14-week-old mice and loaded onto HPTLC plates. Both
Hexb–/–FcRγ–/– and
Hexb–/–FcRγ+/+ mice showed similar levels of GA2 and GM2 storage. M, bovine ganglioside marker.
