A preoperative diagnostic test that distinguishes benign from malignant thyroid carcinoma based on gene expression
J. Clin. Invest. Janete M. Cerutti, et al. 113:1234 doi:10.1172/JCI19617 [Go to this article.]

Figure 2
Quantitative RT-PCR products of three genes with statistically significant expression differences, showing adenoma (A), FTC (C), and normal thyroid (N) tissues and thyroid carcinoma cell lines (CL). The genes DDIT3, ARG2, and ITM1 are expressed in most of the FTCs, the thyroid follicular carcinoma cell line (CL1), the papillary thyroid carcinoma cell line (CL2), and the undifferentiated thyroid carcinoma cell line (CL3), but not in normal tissue and most FTAs. Case 6 (A6) expressed ARG2 and ITM1 and was misclassified according to our class-predicted genes. Universal Human Reference RNA (UHR) was used as a control. Ribosomal protein 8 was used as a calibrator gene. The 100-bp DNA ladder (M) is shown in the far left and far right lanes. The results are shown in triplicate, and the numbers correspond to cases analyzed (Table 2). The product sizes and PCR conditions are described in Methods and summarized in Supplemental Table 1. Negative control (NC) consists of PCR mix and primers, but no template.