Autoreactive T cell responses show proinflammatory polarization in diabetes but a regulatory phenotype in health
J. Clin. Invest. Sefina Arif, et al. 113:451 doi:10.1172/JCI19585 [Go to this article.]

Figure 5
Persistence of T cell responses. (a–d) Blood samples were available from four patients on two occasions 15–23 weeks apart for detection of IFN-γ T cell response using ELISPOT analysis. Shown are mean (± SEM) spots per well for the first and second samples. (a–c) Second samples were tested only against selected peptides positive in the first sample. (d) Two peptides for retesting were selected at random. Background (peptide diluent plus media alone) is represented by the open squares, and the dashed horizontal line represents the cut-off for positivity in each assay (SI ≥ 3). (a) The patient responds to IA-2_752–775 (triangles) on both occasions. (b) The patient responds to IA-2_709–735 (inverted triangles) in both samples, but the response to IA-2_853–872 (circles) declines. (c) The patient responds to IA-2_752–775, IA-2_709–735, and IA-2_793–817 (diamonds) on both occasions. (d) The patient shows no response to IA-2_709–735 or IA-2_752–775 in either sample.