Autoreactive T cell responses show proinflammatory polarization in diabetes but a regulatory phenotype in health
J. Clin. Invest. Sefina Arif, et al. 113:451 doi:10.1172/JCI19585 [Go to this article.]

Figure 4
Cytokine ELISPOT responses to islet peptides are mediated by CD4 T cells. (a and b) Representative analyses from two T1DM patients positive (SI > 3.0) for IFN-γ responses to the IA-2 peptides 709–735 (triangles), 752–775 (squares), and PI peptide C19-A3 (circles), in which peptides were cultured with entire PBMCs or PBMCs immunomagnetically depleted of CD4 T cells before the ELISPOT analysis. (a) Background (medium alone) responses for the patients were 5.3 ± 1.9 spots per well (mean ± SEM) and (b) 1.3 ± 0.6 spots per well. In both subjects positive responses are reduced to background levels by CD4 depletion.