Autoreactive T cell responses show proinflammatory polarization in diabetes but a regulatory phenotype in health
J. Clin. Invest. Sefina Arif, et al. 113:451 doi:10.1172/JCI19585 [Go to this article.]

Figure 2
(a) Determining a cut-off value for assigning positive and negative ELISPOT responses. Graph represents a ROC plot showing assay diagnostic sensitivity (proportion of true positive tests) against specificity (one minus proportion of false positives) following detection of IFN-γ ELISPOT responses to IA-2 and PI peptides in 36 patients with T1DM and 14 nondiabetic control subjects. For each of various possible cut-off values, the sensitivity (proportion of T1DM cases positive) is plotted against one minus specificity representing the proportion of controls that are positive. SIs were calculated as the ratio of the mean response in the presence of peptide to the mean response in the presence of diluent alone. By convention, we selected the cut-off value that provides an operating position nearest to that of the “perfect test” (i.e., closest approximation to 100% sensitivity and 100% specificity), which was SI ≥ 3.0. (b–g) Representative cytokine ELISPOT responses. (b) Representative strong IFN-γ response to IA-2 peptide compared with background response to (c) diluent alone in a patient with T1DM; (d) representative moderate IFN-γ response to PI peptide compared with background response to (e) diluent alone in a patient with T1DM; (f) representative IL-10 response to IA-2 peptide compared with background response to (g) diluent alone in a non-diabetic control subject.