Autoreactive T cell responses show proinflammatory polarization in diabetes but a regulatory phenotype in health
J. Clin. Invest. Sefina Arif, et al. 113:451 doi:10.1172/JCI19585 [Go to this article.]

Figure 1
Reproducibility of cytokine ELISPOT analyses. (a) Intra-assay variability. The same PBMC preparation has been analyzed 12 times on the same sample plate. IFN-γ spot number per well (300,000 cells) is shown for medium and in the presence of 100 ng/ml tetanus toxoid. The intra-assay coefficient of variation for the 12 repeated analyses is 12.3%. (b) Inter-assay variability. The same donor has been venesected on seven separate occasions. IFN-γ spot number per well (300,000 cells) is shown for medium (black bars) and in the presence of 100 ng/ml tetanus toxoid (white bars) for each time point 1–7, representing (time point 1) November 2002, (time points 2–4) three occasions in August 2003, and (time points 5–7) three occasions in September 2003. The interassay coefficient of variation for the seven repeated analyses is 10.2%.