Site and mechanism of leptin action in a rodent form of congenital lipodystrophy
J. Clin. Invest. Esra Asilmaz, et al. 113:414 doi:10.1172/JCI19511 [Go to this article.]

Figure 3
Computational analyses of the hepatic transcription profile after icv and subcutaneous leptin treatment. Cluster analysis, correlation analysis, and the distribution of correlations of liver gene expression from mice treated with icv leptin versus icv PBS and subcutaneous leptin versus subcutaneous PBS are shown. (a) Standard hierarchical clustering of 30 microarray experiments, including icv leptin and subcutaneous leptin treatments, show that the transcription profiles of icv and subcutaneous leptin are more similar to each other than to any other sample. (b) A pairwise comparison of icv and subcutaneous leptin gene expression is shown for genes regulated by both treatments (the intersection set). In the first quadrant, gene expression is increased in subcutaneous treatment and decreased in icv treatment; in the second quadrant, gene expression is increased in both treatments; in the third quadrant, gene expression is decreased in both treatments; and in the fourth quadrant, gene expression is decreased in subcutaneous treatment and increased in icv treatment. In almost all cases, gene expression is similarly regulated by both treatments. ρ denotes the correlation value. (c) The logarithm of fold changes of subcutaneous leptin versus PBS and icv leptin versus PBS for genes regulated by either treatment (the union set) is shown. (d) The distribution of correlations of 3,962 pairwise comparisons from our database is shown. The arrow shows the correlation of the icv leptin versus PBS treatment and the subcutaneous leptin versus PBS treatment. This shows that gene expression after icv and subcutaneous leptin is more highly correlated than for the other comparisons (P < 0.002).