Mitochondrial DNA mutations in human colonic crypt stem cells
J. Clin. Invest. Robert W. Taylor, et al. 112:1351 doi:10.1172/JCI19435 [Go to this article.]

Figure 2
Partial respiratory chain deficiency in some crypts and immunocytochemistry of cytochrome c oxidase deficiency. (a) Reconstructed images were produced from 59 transverse sections (8 μm) covering the full thickness of the colonic lamina propria. Representative images of the sections reacted for cytochrome c oxidase and succinate dehydrogenase are shown in the panel on the extreme right, with the reconstructed crypt arrowed. Scale bar: 50 μm. The position of each image corresponds to the approximate position in the adjacent reconstructed images. The images of the reconstructed colonic crypt are each rotated clockwise through 45°C to show a continuous ribbon of cytochrome c oxidase–deficient cells originating at the crypt base. (b–d) Representative serial transverse sections of colonic crypts showing immunoreactivity of cytochrome c oxidase subunit I (b), immunoreactivity of cytochrome c oxidase subunit IV (c), and dual cytochrome c oxidase/succinate dehydrogenase histochemistry (d). Scale bar: 50 μm (b–d).