High vaccination efficiency of low-affinity epitopes in antitumor immunotherapy
J. Clin. Invest. David-Alexandre Gross, et al. 113:425 doi:10.1172/JCI19418 [Go to this article.]

Figure 5
Absence of autoimmune reactivity against TERT-expressing normal cells and tissues in HHD mice vaccinated with p572Y. (a) Histological analysis of TERT-expressing normal tissues in HHD mice vaccinated with the control gp100₁₅₄ or the p572Y peptide. TERT-expressing spleen (SP), lymph nodes (LN), gut (PP), thymus (TH), and liver (LI) were histologically examined 6 months later. H&E staining. (b) Spleen cells from naive HHD mice vaccinated with gp100₁₅₄ (control) or with the p572Y peptide 6 months earlier were stained with anti-B220–APC, anti- IgD–FITC, anti-IgM–PE. The double anti-IgD and anti-IgM analysis was evaluated for the B220⁺ cell population. (c) IgM and IgG were measured by ELISA in the serum of control HHD mice (white bars) or HHD mice vaccinated with the p572Y peptide 6 months earlier (black bars).