High vaccination efficiency of low-affinity epitopes in antitumor immunotherapy
J. Clin. Invest. David-Alexandre Gross, et al. 113:425 doi:10.1172/JCI19418 [Go to this article.]

Figure 4
Susceptibility to cell lysis by CTL₅₇₂ correlates with telomerase activity in vitro. (a) CTL₅₇₂ line was tested against LPS-activated B cells from HHD (filled circles) and C57BL6 (open circles) mice. E/T ratio, effector/target ratio. (b) Quantification of telomerase activity in normal cells and in tumor cell lines. Telomerase activity was measured by real-time quantitative TRAP assay. Results are calculated from the HL60 cell standard curve and expressed in arbitrary units (a.u.) of telomerase activity defined as the activity of 10³ HL60 cells. Experiments were done in duplicate, and results are expressed as mean ± SD. SDs are always less than 1% of the mean. Mononuclear cells were obtained from HHD bone marrow and LPS or ConA blasts from HHD-activated splenocytes.