Targeted disruption of TGF-β1/Smad3 signaling protects against renal tubulointerstitial fibrosis induced by unilateral ureteral obstruction
J. Clin. Invest. Misako Sato, et al. 112:1486 doi:10.1172/JCI19270 [Go to this article.]

Figure 2
In situ hybridization of α-SMA, Snail, and TGF-β1. (a–d) De novo expression of Snail (a) and α-SMA (c) mRNA in the renal tubular epithelial cells of WT mice at day 7 after UUO. There are no positive signals for Snail (b) or α-SMA (d) mRNA in Smad3-null (KO) counterparts. (e and f) Signals for TGF-β1 mRNA in WT (e) and KO (f) mice at day 14 after UUO. Insets, negative controls reacted with sense probe. Counterstained in nuclear fast red solution. Scale bars: 20 μm. Similar results were obtained from three additional experiments.