Targeted disruption of TGF-β1/Smad3 signaling protects against renal tubulointerstitial fibrosis induced by unilateral ureteral obstruction
J. Clin. Invest. Misako Sato, et al. 112:1486 doi:10.1172/JCI19270 [Go to this article.]

Figure 1
Smad3-null mice maintain the renal architecture after UUO and have reversed EMT. (a) Obstructed kidneys from WT and Smad3-null (KO) mice at day 14 after UUO. (b and c) Hematoxylin and eosin staining of the obstructed kidneys at day 14 after UUO in WT (b) and KO (c) mice. Scale bars: 20 μm. (d–g) Dual immunofluorescence of E-cadherin (green) and α-SMA (red) in obstructed kidneys of WT (d and e) and KO (f and g) mice at day 7 (d and f) and day 14 (e and g) after UUO. DAPI (blue) was used for nuclear staining. Scale bars: 20 μm. (h) Immunoblot of E-cadherin (E-cad) and α-SMA with extracted proteins from kidneys of WT and KO mice with UUO and sham-operated WT mice (Sham). (i) Northern blot of Snail mRNA in kidneys of WT and KO mice with UUO and sham-operated counterparts (Sham).