The pituitary adenylate cyclase–activating polypeptide is a physiological inhibitor of platelet activation
J. Clin. Invest. Kathleen Freson, et al. 113:905 doi:10.1172/JCI19252 [
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Figure 6Decreased levels of PACAP and platelet function. (A) Platelet aggregation induced with a low concentration of ADP (1.25 and 2.5 μM) using PRP from mice injected with the indicated antibodies. (B) Platelet aggregation induced with a low concentration of collagen (0.2 μg/ml) using PRP from mice injected with the indicated antibodies. Platelet aggregation was performed in hirudinized PRP pooled from five mice of each group, normalized to 250 × 10
9 platelets per liter. (C) Platelet aggregation induced with the indicated concentrations of collagen using citrated PRP from either normal (+/+) or PACAP-deficient (–/–) mice. Platelet aggregation was performed in PRP pooled from three mice in each group, normalized to 250 × 10
9 platelets per liter. These tracings were representative of two independent experiments. The difference in base-line response to collagen between Figure
5E and Figure
6C could be related to the different anticoagulants used or the mouse strains. polycl., polyclonal; monocl., monoclonal.
