The pituitary adenylate cyclase–activating polypeptide is a physiological inhibitor of platelet activation
J. Clin. Invest. Kathleen Freson, et al. 113:905 doi:10.1172/JCI19252 [Go to this article.]

Figure 5
Increased levels of PACAP and platelet function. (A) PACAP levels detected by ELISA in plasma (circles) or serum (squares) pooled from three mice. (B) Southern blot analysis of tailsnip DNA. (C) Immunoblot analysis of the prepro-protein of PACAP (20 kDa) in washed platelet releasates pooled from five wild-type versus five PACAP-overexpressing (PACAP-Tg9) mice. (D) PACAP levels detected by ELISA in 0.00125 μl plasma (gray bars) or 0.00125 μl serum (black bars) pooled from three wild-type versus three PACAP-Tg9 mice, performed in duplicate. (E) Left panel: The mean bleeding time ± SD for either wild-type or PACAP-Tg9 mice (ten animals in each group; unpaired t test; P = 0.0001). Right panel: The collagen-induced (5 μg/ml) platelet aggregations in hirudinized PRP from two wild-type and two PACAP-Tg9 mice are shown.