The pituitary adenylate cyclase–activating polypeptide is a physiological inhibitor of platelet activation
J. Clin. Invest. Kathleen Freson, et al. 113:905 doi:10.1172/JCI19252 [
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Figure 2Adenylyl cyclase activity. (A) Measurements of cAMP levels under basal conditions were performed in duplicate (left panel) or after stimulation with iloprost (1 ng/ml) for various time intervals (right panel) in platelets from VI:1 (squares) or an unrelated control (asterisks). (B) Measurements of cAMP levels under basal conditions, performed in duplicate (left panel) or after stimulation with isoproterenol (1 μM) for various time intervals (right panel) in fibroblasts from VI:1 (squares), a patient with a complete trisomy 18 (T18; triangles), or an unrelated control (asterisks). All cAMP measurements were performed in the presence of the phosphodiesterase inhibitor IBMX (100 μM).
