PKCβ regulates ischemia/reperfusion injury in the lung
J. Clin. Invest. Tomoyuki Fujita, et al. 113:1615 doi:10.1172/JCI19225 [Go to this article.]

Figure 4
I/R induces Egr-1 and procoagulant and proinflammatory molecules in the lung: effect of PKCβ. Mice underwent left lung I/R or no instrumentation. Mice were sacrificed and total RNA was isolated from the lung and subjected to Northern analysis (20 ∝g/lane) with ³²P-labeled cDNA probes for Egr-1 (A), tissue factor (TF) (J), PAI-1 (K), IL-1β (L), MIP-2 (M), ICAM-1 (N), or β-actin (as internal control). Real-time PCR analysis of total RNA for Egr-1 expression was performed on I/R and uninstrumented lungs from WT mice fed vehicle or ruboxistaurin chow (B). Immunohistochemical analysis of Egr-1 expression in murine lung from uninstrumented (C) or I/R (D) PKCβ^+/+ mice and from uninstrumented (E) or I/R (F) PKCβ^–/– mice. Ctrl, control. Scale bar in F: 50 ∝M. I/R lungs from PKCβ^+/+ mice were immunofluorescence double stained with an anti–Egr-1 antibody (red) (G) and an anti-MP antibody (F4/80, green) (H). The merging of G (Egr-1) and H (F4/80) is shown in I. Arrows in G and arrowheads in H indicate dually stained cells. Original magnification in G–I, ∞1,000. The units for the y axes of A, B, and J–N are fold increase.