Revascularization of ischemic tissues by PDGF-CC via effects on endothelial cells and their progenitors
J. Clin. Invest. Xuri Li, et al. 115:118 doi:10.1172/JCI19189 [
Go to this article.]
Figure 3Effects of PDGF-CC on endothelial progenitors. (
A) PDGF-CC treatment increased EPC mobilization from day 2 to day 5 after hind limb ischemia but did not affect EPC mobilization in normal conditions. *
P < 0.05. Values are mean ± SEM of 10 mice. (
B–
D) Double labeling of EPCs for PDGF-Rα (green in
B) and Dil-ac-LDL (red in
C), showing coexpression (yellow in
D). (
E–
P) After 2 weeks of stimulation, both PDGF-CC and VEGF induced the expression of EC surface markers CD144 (VE-cadherin) and CD31 (PECAM), while vehicle-treated cells remained negative. Only PDGF-CC induced prominent α-SMA expression, while cells treated with VEGF or vehicle displayed background levels of α-SMA expression. Unstained cells in
E–
G show that both VEGF and PDGF-CC promoted stem/progenitor cell adherence.
