Differential regulation of CCL21 in lymphoid/nonlymphoid tissues for effectively attracting T cells to peripheral tissues
J. Clin. Invest. James C. Lo, et al. 112:1495 doi:10.1172/JCI19188 [Go to this article.]

Figure 3
CCL21 is differentially regulated in the spleen and lung. (a) LT signals are essential for spleen but not lung CCL21. ELISA for CCL21 was performed on spleen and lung homogenates prepared from various LT-deficient mice. Each sample was normalized by tissue weight. (b) Differential requirements for NF-κB and lymphocytes in the expression of CCL21 in the spleen and lung. ELISA for splenic and lung CCL21 from various NF-κB–deficient and Rag^–/– mice, calculated as in a. Each symbol represents data from a single mouse. (c, d) LT selectively regulates the expression of CCL21-Ser but not CCL21-Leu. Real-time PCR analysis was performed on the spleen and lung of WT and LTα^–/– mice with conditions specific for CCL21-Ser (c) and CCL21-Leu (d). The columns and bars represent the mean ± SD.