Differential regulation of CCL21 in lymphoid/nonlymphoid tissues for effectively attracting T cells to peripheral tissues
J. Clin. Invest. James C. Lo, et al. 112:1495 doi:10.1172/JCI19188 [Go to this article.]

Figure 2
Lymphotoxin regulates splenic chemokine expression. (a, b) RNA from WT and LTα^–/– spleen (a) and lung (b) were subjected to chemokine gene array. All signals were normalized to β-actin, which was set to 10,000. Asterisks indicate that no signal above background was detected. RNA from at least three mice was pooled together. A representative blot from two independent experiments is shown. (c, d) LT regulates splenic but not pulmonary CCL21. OCT-embedded (Sakura Finetek, Torrance, California, USA) spleen (c) sections (×100) were stained for CCL21 (blue) and B220 (brown). Formalin-fixed lung (d) sections (×200) were stained for CCL21 (red) and counterstained with hematoxylin. A representative staining from more than five experiments is shown. TARC, thymus and activation-regulated chemokine; I-TAC, IFN-inducible T cell α-chemoattractant; MIG, monokine induced by IFN-γ; MIP, macrophage inflammatory protein.