TNF-α is a critical negative regulator of type 1 immune activation during intracellular bacterial infection
J. Clin. Invest. Anna Zganiacz, et al. 113:401 doi:10.1172/JCI18991 [Go to this article.]

Figure 4
Increased frequency of antigen-specific, IFN-γ–releasing T cells in rBCG-infected TNF^–/– hosts. (a) ELISPOT at day 14. (b) ELISPOT at day 27. At days 14 and 27, isolated total lymphocytes from spleens were cultured in the presence or absence of mycobacterial antigens (M.tb CF) and subject to Elispot assay to determine the relative frequency of mycobacterial antigen-specific T cells. Results are expressed as the average number of antigen-specific T cells per half million cells from duplicate wells per condition, representative of two independent experiments.