Bone marrow–derived progenitor cells in pulmonary fibrosis
J. Clin. Invest. Naozumi Hashimoto, et al. 113:243 doi:10.1172/JCI18847 [
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Figure 6Chemokine and chemokine receptor expression, and fibroblast migration. Lung RNA from saline-treated or BLM-treated mice were obtained at the indicated time points (days after saline or BLM treatment, with day 0 indicating pretreatment values) and analyzed for SDF-1α (
a) and SLC (
b) mRNA using real-time PCR. Data shown at each time point represent the means ± SD from six BLM-treated or saline-treated mice, respectively, and are representative of two independent experiments. (
c) Results of RT-PCR analysis for CXCR4 and CCR7 mRNA in cultured BLF or SLF. The left panel in
c shows a representative electropherogram of the indicated products using RNA samples from: spleen (lane 1), BLF (lanes 2–4), and SLF (lanes 5–7). The right panel in
c summarizes the quantitative results after normalization to the GAPDH signal. Data shown represent the means ± SD (
n = 3), and are representative of three independent experiments. BLF were analyzed for migratory activity toward the indicated chemokine (
d). Additions to the upper (where cells were loaded) or lower chamber were as indicated. Both SLC and SDF-1α were chemotactic for lung fibroblasts, and SDF-1α was also weakly chemokinetic. Data shown represent means ± SD. The experiment was repeated once with similar results. Asterisks signify statistically significant difference (
P < 0.05) between the two groups indicated by connecting lines above the respective bars.
