Bone marrow–derived progenitor cells in pulmonary fibrosis
J. Clin. Invest. Naozumi Hashimoto, et al. 113:243 doi:10.1172/JCI18847 [
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Figure 5Characterization of cultured lung fibroblasts. Lung fibroblasts isolated from BLM-treated GFP BM chimera mice were analyzed by fluorescence microscopy (
a–
f). The cells showed typical fibroblast morphology, many being stellate or spindle-shaped (
a). An average 80% of these cells expressed GFP (green fluorescence in
a, at ×100; inset at ×400). Cells were stained with both anti-GFP (green) and anti–Col I (red) antibodies in
b–
d. The same microscopic field was photographed with the green (
b) or red (
c) filter only, or both simultaneously (
d). Colocalization of both GFP and Col I expression resulted in a yellow color in
d. Inset in
d shows the cells stained with anti-GFP antibody (green) and isotype-matched control IgG for Col I (red). Cells were also stained with both anti-GFP (green) and anti–α-SMA (red) antibodies (
e). Colocalization of GFP and α-SMA should appear yellow, but the two α-SMA
+ cells in this field did not appear to express GFP (
e). Finally, cells were also stained with anti-GFP (green) and anti-TERT (red) antibodies. Colocalization of GFP and TERT appeared yellow, and most of the cells in this field expressed both TERT and GFP (
f). Magnification was ×200 for
b–
f. A representative example of at least three independent experiments is shown.