Bone marrow–derived progenitor cells in pulmonary fibrosis
J. Clin. Invest. Naozumi Hashimoto, et al. 113:243 doi:10.1172/JCI18847 [Go to this article.]

Figure 4
Flow-cytometric analysis of whole-lung cells from GFP BM chimera mice. Whole-lung cells were isolated from BLM-treated (a and c) or saline-treated (b and d) GFP BM chimera mice at day 21 after BLM or saline treatment. Following the appropriate immunostaining, the cells were analyzed by flow cytometry for GFP and Col I expression (c and d) after gating on intact live cells (region indicated by R1) according to side scatter (shown in logarithmic scale, ssLOG) and forward scatter (FS) (a and b). Results of analysis of cells treated with isotype-matched control IgG for the anti–Col I antibody are shown in the insets in c and d. Furthermore, the GFP⁺ cells from these BLM-treated mice were analyzed for Col I and F4/80 or Mac-3 expression after gating on GFP⁺ cells in the R1 region (region indicated by R2 in the inset in e) (e and f). The Col I⁺ and F4/80⁺ cells represented 2.7% ± 0.76% of GFP⁺ cells (e). The Col I⁺ and Mac-3⁺ cells represented 5.5% ± 0.4% of GFP⁺ cells (f). Inset in f shows the cells stained with isotype-matched control IgG. Representative runs are shown for each group from a total of eight BLM-treated or saline-treated GFP BM chimera mice, respectively. The quantitative results are summarized in Table 1.