Bone marrow–derived progenitor cells in pulmonary fibrosis
J. Clin. Invest. Naozumi Hashimoto, et al. 113:243 doi:10.1172/JCI18847 [Go to this article.]

Figure 3
Morphology of BLM-induced lung fibrosis in BM chimera mice. Representative lung sections from BLM-treated (a–c) or saline-treated (d–f) GFP BM chimera mice were evaluated at day 28 after BLM or saline treatment. The H&E-stained sections revealed severe distortion of lung alveolar architecture due to extensive fibrosis (a and b, at ×40 and ×200, respectively). (c) Numerous GFP⁺ cells in densely cellular fibrotic lesions were easily identifiable by fluorescence microscopy in BLM-treated lungs. ×200. In contrast, H&E-stained lung sections from saline-treated GFP BM chimera mice showed normal lung architecture (d and e, at ×40 and ×200, respectively), with a few scattered GFP⁺ cells visualized by fluorescence microscopy (f, at ×200). Insets in c and f showed the light-microscopic appearance of the respective sections examined by fluorescence microscopy. ×200.