Methylguanine methyltransferase–mediated in vivo selection and chemoprotection of allogeneic stem cells in a large-animal model
J. Clin. Invest. Tobias Neff, et al. 112:1581 doi:10.1172/JCI18782 [Go to this article.]

Figure 1
In vivo selection in dog G069 after two cycles of O6BG and BCNU separated by 1 year. (a–c) Flow-cytometric analysis. (a) Percent EGFP-positive granulocytes (open circles) and lymphocytes (filled squares) from peripheral blood. (b) Percent EGFP-positive red blood cells (open circles) and platelets (filled squares) from peripheral blood. (c) Percent EGFP-positive CD34+ cells from bone marrow (open circles). (d) Quantification of provirus in leukocytes from peripheral blood by TaqMan PCR (open circles). (e) Platelet counts (Plt) in the time periods following the first and second cycle of O6BG and BCNU. The arrows indicate the two cycles of drug administration.