RAGE^–/– mice and WT mice treated with sRAGE are protected against septic shock induced by CLP. (A) Age- and sex-matched WT and RAGE^–/– mice were subjected to CLP or sham operation and were monitored for survival. WT indicates C57BL/6 ∞ 129/Sv mice. A summary of four repeated experiments is shown. One to two sham-operated mice were included in each single experiment (data not shown) and did not show overt signs of disease. WT, filled squares; RAGE^–/–, open circles. (B) Therapeutic effect of sRAGE treatment. CLP was performed in age- and sex-matched C57BL/6 mice. Mice received repeated intraperitoneal injections of sRAGE or solvent (LPS-free 0.9% NaCl) immediately after CLP (150 ∝g sRAGE/mouse) and 6, 12, 24, and 36 hours thereafter (70 ∝g sRAGE/mouse). Two repeated experiments are summarized. WT treated with solvent, filled triangles; WT treated with sRAGE, asterisks. (C) Age- and sex-matched WT and Tie2RAGE mice were subjected to CLP and were monitored for survival. WT indicates transgene-negative littermates. A summary of two repeated experiments is shown. One to two sham-operated mice were included in each single experiment (data not shown) and did not show overt signs of disease. WT, filled squares; Tie2RAGE, open triangles.