Cathepsin V is involved in the degradation of invariant chain in human thymus and is overexpressed in myasthenia gravis
J. Clin. Invest. Eva Tolosa, et al. 112:517 doi:10.1172/JCI18028 [Go to this article.]

Figure 2
Specificity of mAb33/1 and mAb33/2 for human Cats V and L. (a) Western blot analysis of recombinant human Cats V and L using mAb33/1 and mAb33/2. Both enzymes, 0.05 μg, were loaded onto 12% SDS Tris-glycine gels and antibody dilutions of 1:5000 were used. The mAb33/2 cross reacts with both proteases, whereas mAb33/1 only recognizes Cat L. (b) The mAb33/1 and mAb33/2 recognize Cat L in human spleen tissue sections and are indistinguishable in their sensitivity toward Cat L antigen in paraffin-embedded tissue section. The ABC staining kit, Super Sensitive Ready-To-Use Detection System (Biogenex), was used for immunohistochemical protein localization.