Regulatory functions of CD8⁺CD28^– T cells in an autoimmune disease model
J. Clin. Invest. Nader Najafian, et al. 112:1037 doi:10.1172/JCI17935 [Go to this article.]

Figure 6
IFN-γ production of splenocytes in response to MOG peptide in vitro. MOG p35-55–specific IFN-γ–producing cells were measured by ELISPOT in cultures of splenocytes harvested on day 14 from: (a) CD28^–/– mice (gray bars), CD28^–/– mice depleted from CD8⁺ T cells in vivo by mAb (white bars) and CD28^–/–CD8^–/– mice (black bars). (b) WT mice (gray bars), WT mice depleted from CD8⁺ T cells in vivo by mAb (white bars), and CD8^–/– mice (black bars). The y axis represents the number of positive cells per one million cells. Purified protein derivative (PPD) is used at a concentration of 100 μg/ml. (c) WT mice (gray bars), STAT4^–/– mice (white bars), and STAT4^–/–mice depleted from CD8⁺ T cells in vivo by mAb (black bars). (d) Expansion of IFN-γ–producing T cells after ex vivo depletion of CD8⁺ T cells: MOG p35-55–specific IFN-γ–producing cells were measured by ELISPOT in cultures of splenocytes harvested on day 14 from CD28^–/– mice before and after CD8⁺ T cell depletion ex vivo by magnetic beads. The frequency of IFN-γ–producing cells is significantly higher after removal of CD8⁺ T cells at all concentrations of MOG or PPD (P < 0.02). s/p, status post.