Regulatory functions of CD8⁺CD28^– T cells in an autoimmune disease model
J. Clin. Invest. Nader Najafian, et al. 112:1037 doi:10.1172/JCI17935 [Go to this article.]

Figure 4
Suppression of EAE by adoptive transfer of CD8⁺CD28^– cells into CD8^–/– mice. (a) 100% purified CD8⁺CD28^– cells were generated from naive CD28^–/– splenocytes and injected into CD8^–/– recipients via the tail vein, as described in Methods. The recipient mice were then immunized with MOG peptide on the same day. The mean daily score for each group (n = 10) is shown on the y axis. The course of CD8^–/– mice (open squares), CD8^–/– recipients of CD8⁺CD28^– cells (open circles), and WT mice (filled squares) is shown. (b) One hundred percent purified CD8⁺CD28⁺ and CD8⁺CD28^– T cells were isolated from spleens of naive WT mice. Approximately one million cells were then injected into each CD8^–/– recipient as described above. The mean daily score for each group (n = 6) is shown on the y axis. CD8⁺CD28^– T cells (open circles) significantly suppress the EAE as compared with the control group (open squares), while CD8⁺CD28⁺ T cells do not show any significant effect on disease course (filled circles). Adoptive transfer of approximately five million CD8⁺CD28^– T cells did not lead to any further suppression of disease (filled squares).